Recombinant:Article Title: Limited activation interdependence and differing patterns of the in vivo effects of IKK1 and IKK2 downstream of NIK.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat polyclonal anti-mouse IgA, HRP Invitrogen Cat#62–6720; RRID:AB_138466 Goat anti-mouse IgG (H + L), HRP Invitrogen Cat#62–6520; RRID:AB_2533947 Rat anti-Mouse CD45R BD Biosciences Cat#550286; RRID:AB_393581 Rabbit monoclonal anti-CD3 Abcam Cat#ab16669; RRID:AB_443425 Cy2-Donkey polyclonal anti-Rat IgG (H + L) Jackson Immunoresearch Cat#712-225-153; RRID:AB_2340674 Cy3-Donkey polyclonal anti-Rabbit IgG (H + L) Jackson Immunoresearch Cat#711-165-152; RRID:AB_2307443 Chemicals, peptides, and recombinant proteins SMI1 MedChemExpress Cat#HY-112433 IKK16 MedChemExpress Cat#HY-13687 MG132 Sigma Cat#M7449 4-Hydroxytamoxifen Sigma Cat#H6278 Primocin Invitrogen Cat#ant-pm-05 Puromycin Invitrogen Cat#ant-pr-1 G418 disulfate Sigma Cat#A1720 jetPEI Polyplus Cat#101-40N Lipofectamine 3000 Thermo Fisher Cat#L3000015 Trypsin-EDTA (0.5%) 10X Gibco Cat#15400054 HANKS buffer Sigma Cat#H6648 MEM Non-Essential Amino Acids Solution (100X) Biological Industries Cat#01-340-1B Sodium Pyruvate Solution 100mM Biological Industries Cat#03-042-1B Chicago Sky Blue 6B Sigma Cat#C8679-25G NuPAGE LDS sample Buffer (4X) Thermo Fisher Cat#NP0007 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Cat#NP0002 NuPAGE® MOPS SDS Running Buffer (20X) Thermo Fisher Cat#NP0001 TG-SDS buffer (10x) Bio-Lab Cat#20522323 Ultra TMB ELISA substrate Thermo Fisher Cat#34028 TNP-KLH SANTA CRUZ Cat#sc-396496 TNP-BAS Biosearch Technologies Cat#T-5050-10 Recombinant human TWEAK Peprotech Cat#310-06-100 Recombinant human TNFα Peprotech Cat#300-01A-10 Poly R (RRRRRRRRRGG) This paper N/A Poly R-X (RRRRRRRRRGGSSPERKATVPEQEL QQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSL) This paper N/A Poly R-X mut (RRRRRRRRRGGSSPERKATVPEQ ELQQLEIEAFANSASQPFSLEEQEQILSCLSIDSLSL) This paper N/A Critical commercial assays SuperScript II Invitrogen Cat#18064014 Oligo(dT)15 Primer Promega Cat#C1101 TaqMan Master Mix Thermo Fisher Cat#AB-4304437 Power SYBR Green PCR Master Mix Thermo Fisher Cat#4367659 Pierce 660 nm protein assay Thermo Fisher Cat#22660 SuperSigna West Pico PLUS Chemiluminescent Substrate Thermo Fisher Cat#34580 WESTAR NOVA 2.0 ECL kit CYANAGEN Cat#XLS071 RNeasy mini kit QIAGEN Cat#/ID: 74104 NucleoSpin RNA mini kit MACHEREY-NAGEL Cat#740955.50 Nunc-Immuno MicroWell 96 well-solid plates Sigma Cat#M9410-1CS MicroAMP optical 384-well reaction plate Applied Biosystems TM Cat#4309849 MicroAmp TM Optical 96-Well Reaction Plate Applied Biosystems TM Cat#N8010560 Homogenizer (Biomasher II) DiagnoCine Cat#FNK-893062 (Continued on next page) Cell Reports 44, 116404, October 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER mPage 4–12% Bis-Tris 10x8 15 well Merck Millipore Cat#MP41G15 mPage 8% Bis-Tris 10x8 15 well Merck Millipore Cat#MP8W15 Deposited data Raw and analyzed data from Western blot assay This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from RNA-seq assay This paper GEO accession number: GSE306633 Affinity-based mass spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from immunostaining This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from circular dichroism spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from flow cytometry analysis This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Experimental models: Cell lines Phoenix AMPHO ATCC Cat#CRL-3213 RRID:CVCL_H716 Phoenix ECO ATCC Cat#CRL-3214 RRID:CVCL_H717 HT29 ATCC Cat# HTB-38 RRID:CVCL_0320 HeLa ATCC Cat#CRL-7923 RRID:CVCL_0030 HEK293T ATCC Cat#CRL-3216 RRID:CVCL_0063 MDF (Mouse dermal fibroblast) This paper N.A.
Enzyme-linked Immunosorbent Assay:Article Title: Limited activation interdependence and differing patterns of the in vivo effects of IKK1 and IKK2 downstream of NIK.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat polyclonal anti-mouse IgA, HRP Invitrogen Cat#62–6720; RRID:AB_138466 Goat anti-mouse IgG (H + L), HRP Invitrogen Cat#62–6520; RRID:AB_2533947 Rat anti-Mouse CD45R BD Biosciences Cat#550286; RRID:AB_393581 Rabbit monoclonal anti-CD3 Abcam Cat#ab16669; RRID:AB_443425 Cy2-Donkey polyclonal anti-Rat IgG (H + L) Jackson Immunoresearch Cat#712-225-153; RRID:AB_2340674 Cy3-Donkey polyclonal anti-Rabbit IgG (H + L) Jackson Immunoresearch Cat#711-165-152; RRID:AB_2307443 Chemicals, peptides, and recombinant proteins SMI1 MedChemExpress Cat#HY-112433 IKK16 MedChemExpress Cat#HY-13687 MG132 Sigma Cat#M7449 4-Hydroxytamoxifen Sigma Cat#H6278 Primocin Invitrogen Cat#ant-pm-05 Puromycin Invitrogen Cat#ant-pr-1 G418 disulfate Sigma Cat#A1720 jetPEI Polyplus Cat#101-40N Lipofectamine 3000 Thermo Fisher Cat#L3000015 Trypsin-EDTA (0.5%) 10X Gibco Cat#15400054 HANKS buffer Sigma Cat#H6648 MEM Non-Essential Amino Acids Solution (100X) Biological Industries Cat#01-340-1B Sodium Pyruvate Solution 100mM Biological Industries Cat#03-042-1B Chicago Sky Blue 6B Sigma Cat#C8679-25G NuPAGE LDS sample Buffer (4X) Thermo Fisher Cat#NP0007 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Cat#NP0002 NuPAGE® MOPS SDS Running Buffer (20X) Thermo Fisher Cat#NP0001 TG-SDS buffer (10x) Bio-Lab Cat#20522323 Ultra TMB ELISA substrate Thermo Fisher Cat#34028 TNP-KLH SANTA CRUZ Cat#sc-396496 TNP-BAS Biosearch Technologies Cat#T-5050-10 Recombinant human TWEAK Peprotech Cat#310-06-100 Recombinant human TNFα Peprotech Cat#300-01A-10 Poly R (RRRRRRRRRGG) This paper N/A Poly R-X (RRRRRRRRRGGSSPERKATVPEQEL QQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSL) This paper N/A Poly R-X mut (RRRRRRRRRGGSSPERKATVPEQ ELQQLEIEAFANSASQPFSLEEQEQILSCLSIDSLSL) This paper N/A Critical commercial assays SuperScript II Invitrogen Cat#18064014 Oligo(dT)15 Primer Promega Cat#C1101 TaqMan Master Mix Thermo Fisher Cat#AB-4304437 Power SYBR Green PCR Master Mix Thermo Fisher Cat#4367659 Pierce 660 nm protein assay Thermo Fisher Cat#22660 SuperSigna West Pico PLUS Chemiluminescent Substrate Thermo Fisher Cat#34580 WESTAR NOVA 2.0 ECL kit CYANAGEN Cat#XLS071 RNeasy mini kit QIAGEN Cat#/ID: 74104 NucleoSpin RNA mini kit MACHEREY-NAGEL Cat#740955.50 Nunc-Immuno MicroWell 96 well-solid plates Sigma Cat#M9410-1CS MicroAMP optical 384-well reaction plate Applied Biosystems TM Cat#4309849 MicroAmp TM Optical 96-Well Reaction Plate Applied Biosystems TM Cat#N8010560 Homogenizer (Biomasher II) DiagnoCine Cat#FNK-893062 (Continued on next page) Cell Reports 44, 116404, October 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER mPage 4–12% Bis-Tris 10x8 15 well Merck Millipore Cat#MP41G15 mPage 8% Bis-Tris 10x8 15 well Merck Millipore Cat#MP8W15 Deposited data Raw and analyzed data from Western blot assay This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from RNA-seq assay This paper GEO accession number: GSE306633 Affinity-based mass spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from immunostaining This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from circular dichroism spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from flow cytometry analysis This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Experimental models: Cell lines Phoenix AMPHO ATCC Cat#CRL-3213 RRID:CVCL_H716 Phoenix ECO ATCC Cat#CRL-3214 RRID:CVCL_H717 HT29 ATCC Cat# HTB-38 RRID:CVCL_0320 HeLa ATCC Cat#CRL-7923 RRID:CVCL_0030 HEK293T ATCC Cat#CRL-3216 RRID:CVCL_0063 MDF (Mouse dermal fibroblast) This paper N.A.
SYBR Green Assay:Article Title: Limited activation interdependence and differing patterns of the in vivo effects of IKK1 and IKK2 downstream of NIK.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat polyclonal anti-mouse IgA, HRP Invitrogen Cat#62–6720; RRID:AB_138466 Goat anti-mouse IgG (H + L), HRP Invitrogen Cat#62–6520; RRID:AB_2533947 Rat anti-Mouse CD45R BD Biosciences Cat#550286; RRID:AB_393581 Rabbit monoclonal anti-CD3 Abcam Cat#ab16669; RRID:AB_443425 Cy2-Donkey polyclonal anti-Rat IgG (H + L) Jackson Immunoresearch Cat#712-225-153; RRID:AB_2340674 Cy3-Donkey polyclonal anti-Rabbit IgG (H + L) Jackson Immunoresearch Cat#711-165-152; RRID:AB_2307443 Chemicals, peptides, and recombinant proteins SMI1 MedChemExpress Cat#HY-112433 IKK16 MedChemExpress Cat#HY-13687 MG132 Sigma Cat#M7449 4-Hydroxytamoxifen Sigma Cat#H6278 Primocin Invitrogen Cat#ant-pm-05 Puromycin Invitrogen Cat#ant-pr-1 G418 disulfate Sigma Cat#A1720 jetPEI Polyplus Cat#101-40N Lipofectamine 3000 Thermo Fisher Cat#L3000015 Trypsin-EDTA (0.5%) 10X Gibco Cat#15400054 HANKS buffer Sigma Cat#H6648 MEM Non-Essential Amino Acids Solution (100X) Biological Industries Cat#01-340-1B Sodium Pyruvate Solution 100mM Biological Industries Cat#03-042-1B Chicago Sky Blue 6B Sigma Cat#C8679-25G NuPAGE LDS sample Buffer (4X) Thermo Fisher Cat#NP0007 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Cat#NP0002 NuPAGE® MOPS SDS Running Buffer (20X) Thermo Fisher Cat#NP0001 TG-SDS buffer (10x) Bio-Lab Cat#20522323 Ultra TMB ELISA substrate Thermo Fisher Cat#34028 TNP-KLH SANTA CRUZ Cat#sc-396496 TNP-BAS Biosearch Technologies Cat#T-5050-10 Recombinant human TWEAK Peprotech Cat#310-06-100 Recombinant human TNFα Peprotech Cat#300-01A-10 Poly R (RRRRRRRRRGG) This paper N/A Poly R-X (RRRRRRRRRGGSSPERKATVPEQEL QQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSL) This paper N/A Poly R-X mut (RRRRRRRRRGGSSPERKATVPEQ ELQQLEIEAFANSASQPFSLEEQEQILSCLSIDSLSL) This paper N/A Critical commercial assays SuperScript II Invitrogen Cat#18064014 Oligo(dT)15 Primer Promega Cat#C1101 TaqMan Master Mix Thermo Fisher Cat#AB-4304437 Power SYBR Green PCR Master Mix Thermo Fisher Cat#4367659 Pierce 660 nm protein assay Thermo Fisher Cat#22660 SuperSigna West Pico PLUS Chemiluminescent Substrate Thermo Fisher Cat#34580 WESTAR NOVA 2.0 ECL kit CYANAGEN Cat#XLS071 RNeasy mini kit QIAGEN Cat#/ID: 74104 NucleoSpin RNA mini kit MACHEREY-NAGEL Cat#740955.50 Nunc-Immuno MicroWell 96 well-solid plates Sigma Cat#M9410-1CS MicroAMP optical 384-well reaction plate Applied Biosystems TM Cat#4309849 MicroAmp TM Optical 96-Well Reaction Plate Applied Biosystems TM Cat#N8010560 Homogenizer (Biomasher II) DiagnoCine Cat#FNK-893062 (Continued on next page) Cell Reports 44, 116404, October 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER mPage 4–12% Bis-Tris 10x8 15 well Merck Millipore Cat#MP41G15 mPage 8% Bis-Tris 10x8 15 well Merck Millipore Cat#MP8W15 Deposited data Raw and analyzed data from Western blot assay This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from RNA-seq assay This paper GEO accession number: GSE306633 Affinity-based mass spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from immunostaining This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from circular dichroism spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from flow cytometry analysis This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Experimental models: Cell lines Phoenix AMPHO ATCC Cat#CRL-3213 RRID:CVCL_H716 Phoenix ECO ATCC Cat#CRL-3214 RRID:CVCL_H717 HT29 ATCC Cat# HTB-38 RRID:CVCL_0320 HeLa ATCC Cat#CRL-7923 RRID:CVCL_0030 HEK293T ATCC Cat#CRL-3216 RRID:CVCL_0063 MDF (Mouse dermal fibroblast) This paper N.A.
Polymerase Chain Reaction:Article Title: Limited activation interdependence and differing patterns of the in vivo effects of IKK1 and IKK2 downstream of NIK.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat polyclonal anti-mouse IgA, HRP Invitrogen Cat#62–6720; RRID:AB_138466 Goat anti-mouse IgG (H + L), HRP Invitrogen Cat#62–6520; RRID:AB_2533947 Rat anti-Mouse CD45R BD Biosciences Cat#550286; RRID:AB_393581 Rabbit monoclonal anti-CD3 Abcam Cat#ab16669; RRID:AB_443425 Cy2-Donkey polyclonal anti-Rat IgG (H + L) Jackson Immunoresearch Cat#712-225-153; RRID:AB_2340674 Cy3-Donkey polyclonal anti-Rabbit IgG (H + L) Jackson Immunoresearch Cat#711-165-152; RRID:AB_2307443 Chemicals, peptides, and recombinant proteins SMI1 MedChemExpress Cat#HY-112433 IKK16 MedChemExpress Cat#HY-13687 MG132 Sigma Cat#M7449 4-Hydroxytamoxifen Sigma Cat#H6278 Primocin Invitrogen Cat#ant-pm-05 Puromycin Invitrogen Cat#ant-pr-1 G418 disulfate Sigma Cat#A1720 jetPEI Polyplus Cat#101-40N Lipofectamine 3000 Thermo Fisher Cat#L3000015 Trypsin-EDTA (0.5%) 10X Gibco Cat#15400054 HANKS buffer Sigma Cat#H6648 MEM Non-Essential Amino Acids Solution (100X) Biological Industries Cat#01-340-1B Sodium Pyruvate Solution 100mM Biological Industries Cat#03-042-1B Chicago Sky Blue 6B Sigma Cat#C8679-25G NuPAGE LDS sample Buffer (4X) Thermo Fisher Cat#NP0007 NuPAGE MES SDS Running Buffer (20X) Thermo Fisher Cat#NP0002 NuPAGE® MOPS SDS Running Buffer (20X) Thermo Fisher Cat#NP0001 TG-SDS buffer (10x) Bio-Lab Cat#20522323 Ultra TMB ELISA substrate Thermo Fisher Cat#34028 TNP-KLH SANTA CRUZ Cat#sc-396496 TNP-BAS Biosearch Technologies Cat#T-5050-10 Recombinant human TWEAK Peprotech Cat#310-06-100 Recombinant human TNFα Peprotech Cat#300-01A-10 Poly R (RRRRRRRRRGG) This paper N/A Poly R-X (RRRRRRRRRGGSSPERKATVPEQEL QQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSL) This paper N/A Poly R-X mut (RRRRRRRRRGGSSPERKATVPEQ ELQQLEIEAFANSASQPFSLEEQEQILSCLSIDSLSL) This paper N/A Critical commercial assays SuperScript II Invitrogen Cat#18064014 Oligo(dT)15 Primer Promega Cat#C1101 TaqMan Master Mix Thermo Fisher Cat#AB-4304437 Power SYBR Green PCR Master Mix Thermo Fisher Cat#4367659 Pierce 660 nm protein assay Thermo Fisher Cat#22660 SuperSigna West Pico PLUS Chemiluminescent Substrate Thermo Fisher Cat#34580 WESTAR NOVA 2.0 ECL kit CYANAGEN Cat#XLS071 RNeasy mini kit QIAGEN Cat#/ID: 74104 NucleoSpin RNA mini kit MACHEREY-NAGEL Cat#740955.50 Nunc-Immuno MicroWell 96 well-solid plates Sigma Cat#M9410-1CS MicroAMP optical 384-well reaction plate Applied Biosystems TM Cat#4309849 MicroAmp TM Optical 96-Well Reaction Plate Applied Biosystems TM Cat#N8010560 Homogenizer (Biomasher II) DiagnoCine Cat#FNK-893062 (Continued on next page) Cell Reports 44, 116404, October 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER mPage 4–12% Bis-Tris 10x8 15 well Merck Millipore Cat#MP41G15 mPage 8% Bis-Tris 10x8 15 well Merck Millipore Cat#MP8W15 Deposited data Raw and analyzed data from Western blot assay This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from RNA-seq assay This paper GEO accession number: GSE306633 Affinity-based mass spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from immunostaining This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from circular dichroism spectrometry This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Raw and analyzed data from flow cytometry analysis This paper Mendeley Data https://doi.org/10.17632/ k7pcdgr73t.1 Experimental models: Cell lines Phoenix AMPHO ATCC Cat#CRL-3213 RRID:CVCL_H716 Phoenix ECO ATCC Cat#CRL-3214 RRID:CVCL_H717 HT29 ATCC Cat# HTB-38 RRID:CVCL_0320 HeLa ATCC Cat#CRL-7923 RRID:CVCL_0030 HEK293T ATCC Cat#CRL-3216 RRID:CVCL_0063 MDF (Mouse dermal fibroblast) This paper N.A.
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